The kit contains a ready-to-use PCR reaction mix with highly-optimized mycoplasma-specific primers, a positive control, and an internal control, all for the simple and efficient discovery of mycoplasma contamination. Samples can be prepared in as little as 10 minutes and results can be easily obtained within just a few hours. Easy to use. Easy to run. Follow a simple protocol with ready-to-use reagents for as little as 10 minutes of preparation. Easy to interpret.
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Time: by Abnova Mycoplasma is a common and serious contamination of cell cultures. Mycoplasma contamination can cause the alteration of the phenotypic cell characteristics. PCR method allows for fast and reliable identification of mycoplasma contamination in cell cultures.
Resazurin, a non-fluorescent indicator dye, is converted to highly red fluorescent resorufin via reduction reactions of metabolically active cells. The amount of fluorescence produced is proportional to the number of living cells.
Calcium forms an Alizarin Red S-calcium complex in a chelation process. This video describes the procedure of Alizarin Red S Staining for osteogenesis. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability.
Each detected signal is visualized as an individual fluorescent dot, these signals can be quantified counted and assigned to a specific subcellular location based on microscopy images. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid.
Covalent attachment of PEG PEGylation to proteins can reduce their immunogenicity, minimize proteolytic cleavage and increase their serum half-life.
PEG has also been attached to small molecules and liposomes for more selective delivery. PEG-modification of superparamagnetic iron oxide and quantum dots can improve their biocompatibility and reduce non-specific uptake.
The second part of the assay uses the Caspase-GloR to measure caspase activity. Learn how to use the biological indicator in this AbVideo. There are also physical and chemical indicators that can be used to ensure decontamination effectiveness of autoclaves. Check out how to use it to maintain the maximum performance of your autoclave machine. There are physical, chemical, and biological indicators that can be used to ensure decontamination effectiveness of autoclaves.
The BSC is tested for bioburden to ensure the adequate protection. To perform a blood smear, two very clean slides are required. Drop a small drop of blood on one of the slides. Pull blood forward across slide. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.
Fixation methods fall generally into two classes: organic solvents and cross-linking reagents. This video uses formaldehyde as a cross-linking reagent to fix cells. The reagent contains a tetrazolium compound MTS. The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at nm. By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of nm.
Nocodazole is one of anticancer drugs that synchronize cells at M-phase. It can interfere with the structure and function of microtubules in interphase and mitotic cells. Kumiko Ui-Tei at the University of Tokyo. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate.
It uses the Orsat method of volumetric analysis involving chemical potassium hydroxide absorption of CO2 gas. Because DNA is hydrophilic, it will not normally pass through membrane.
In order to make these cells readily incorporate foreign DNA, they must first be made "competent" to take up foreign DNA. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well. The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control.
The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present. It inactivates the RNases by the covalent modifications of the histidine residues.
The membranes will have a molecular weight cut-off MWCO. This is the limit of size or range of a protein that can fit through the membrane. It is indispensable for basic biological research and discovery. It is for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane.
It is usually used in molecular biology as a way of introducing some substance into a cell, such as molecular probes, drugs or pieces of coding DNA. Adjuvants are mixed and injected with an antigen to prevent catabolism and help increase the immune response by localizing the antigen for an extended time and attracting the appropriate cells T cells, B cells and APC to interact with it.
EZ-PCR Mycoplasma Test Kit
EZ-PCR™ Mycoplasma Detection Kit