Besides gravimetrical analysis of the ratanhiaphenols I, II and III, the content of these compounds in the herbal drug has never been determined. The developed HPLC method enables the quantification of twelve active lignan derivatives in the roots, and is also suitable for the determination of the constituents in Tinctura Ratanhiae. Separation was achieved on a phenyl-hexyl column material using a solvent gradient consisting of 0. HPLC—MS experiments in positive and negative electrospray ionization mode confirmed identity and peak purity of analytes. Introduction Krameria lappacea Dombey Burdet et Simpson; syn.
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So far, the astringent and antimicrobial properties of its proanthocyanidin constituents are considered to account for the anti-inflammatory effect.
The aim of the present study was to characterize pharmacologically a lipophilic extract of K. The present findings not only support the ethnopharmacological use of K. The roots of Krameria lappacea Dombey Burdet et Simpson syn. Its therapeutic indications comprise mild incidences of inflammation of the mouth and throat such as stomatitis, gingivitis, and pharyngitis. Extracts of the roots, as well as some of the pure constituents, were shown to possess antimicrobial, antioxidant, and photoprotective activities.
From the dichloromethane extract of K. Since rhatany preparations are used mainly topically against infections and inflammation, as a first step a potential topical anti-inflammatory effect in vivo was assessed using a croton oil-induced mouse ear dermatitis model. Since the inflammatory response is a complex process, influenced by a number of different mediators, various molecular targets were evaluated to gain insight into the mode of action of the isolated secondary metabolites.
To evaluate the anti-inflammatory potency of the extract as well as of the isolated compounds, ID50 values were assessed. The lignan derivatives showed ID50 values in the range 0. Table 1.
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